(1-3)-β-D-Glucan Detection Kit (Kinetic Chromogenic Method)

Fungi(1,3)-β-D-glucan Assay Kit

Product information:

(1-3)-β-D-Glucan Detection Kit (Kinetic Chromogenic Method) measures levels of (1-3)-β-D-Glucan by kinetic chromogenic method. The assay is based upon a modification factor G pathway of the Amebocyte Lysate (AL). (1-3)-β-D-Glucan activates Factor G, the activated Factor G converts the inactive proclotting enzyme to the active clotting enzyme, which in turn cleaves pNA from the chromogenic peptide substrate. pNA is a chromophore that absorbs at 405 nm. The rate of OD increase at 405nm of the reaction solution is directly proportional to the concentration of the reaction solution (1-3)-β-D-Glucan. The concentration of (1-3)-β-D-Glucan in the reaction solution can be calculated according to the standard curve by recording the rate of change of the OD value of the reaction solution through optical detection equipment and software.

The highly sensitive, rapid assay assists clinicians in identifying Invasive Fungal Disease (IFD) early in the disease process. The kit has obtained EU CE qualification and can be used for clinical diagnosis.

Immunosuppressed patients are at high risk for developing invasive fungal disease, which is often difficult to diagnose. Affected patient populations include:

Cancer patients undergoing chemotherapy

Stem Cell and Organ Transplant patients

Burn patients

HIV patients

ICU patients

Product parameter:

Assay range: 25-1000 pg/ml

Assay time: 40 minutes, sample pre-treatment: 10 minutes

Note:

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.

Catalog number:

KCG50 (50 tests / kit):  Chromogenic Amebocyte Lysate  1.1mL×5

                   (1-3)-β-D-Glucan standard       1mL×2

                   Reconstitution Buffer         10mL×2

                   Tris Buffer                  6mL×1

                   Sample Treatment Solution A   3mL×1

                   Sample Treatment Solution B   3mL×1

KCG80 (80 tests / kit):  Chromogenic Amebocyte Lysate  1.7mL×5

                   (1-3)-β-D-Glucan standard       1mL×2

                   Reconstitution Buffer         10mL×2

                   Tris Buffer                  6mL×1

                   Sample Treatment Solution A   3mL×1

                   Sample Treatment Solution B   3mL×1

KCG100 (100 tests / kit): Chromogenic Amebocyte Lysate  2.2mL×5

                   (1-3)-β-D-Glucan standard       1mL×2

                   Reconstitution Buffer         10mL×2

                   Tris Buffer                  6mL×1

                   Sample Treatment Solution A   3mL×1

                   Sample Treatment Solution B   3mL×1

Product Condition:

The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.