China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo


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China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)

1. Product Information

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis. 

2. Product Parameter

Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)

                             0.1-1.0EU/ml (assay time about 16 minutes)

3. Product Features and Application

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).

 

Note:

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.

Catalog No.

Description

Kit Contents

Sensitivity EU/ml

EC80545

Bioendo™ EC Endotoxin Test Kit

(End-point Chromogenic Assay,

Diazo Coupling),

80 Tests/Kit

5 Lyophilized Amebocyte Lysate, 1.7ml/vial;

4 Water for BET, 50ml/vial;

5 CSE;

5 Chromogenic Substrate, 1.7ml/vial;

5 Diazo Reagent 1, 10ml/vial;

5 Diazo Reagent 2, 10ml /vial;

5 Diazo Reagent 3, 10ml/vial;

0.1 – 1 EU/ml

EC80545S

0.01 – 0.1 EU/ml;

0.1 – 1 EU/ml

Product Conditions

The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.

 

Does the End point endotoxin test kit need sophisticated microplate reader?

Bioendo EC80545 and EC80545S, can read by regular spectrophotometer. 

Bioendo End point chromogenic endotoxin test kit series:
Endotoxin Free Tube
Pyrogen Free Tips
Pyrogen Free Microplates
normal Microplate reader
Water Bath or Dry Heat Incubator 

Product detail pictures:

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures


Related Product Guide:

Our well-equipped facilities and great excellent command throughout all stages of generation enables us to guarantee total customer fulfillment for China wholesale Chromogenic endotoxin detection - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Tanzania, Iceland, Hyderabad, We maintain long-term efforts and self-criticism, which helps us and improvement constantly. We strive to improve customer efficiency to save costs for customers. We do our best to improve the quality of product. We will not live up to the historic opportunity of the times.
  • The enterprise has a strong capital and competitive power, product is sufficient, reliable, so we have no worries on cooperating with them.
    5 Stars By Daphne from Oman - 2018.07.27 12:26
    Factory equipment is advanced in the industry and the product is fine workmanship, moreover the price is very cheap, value for money!
    5 Stars By Myra from Bolivia - 2017.07.07 13:00

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