Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo


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Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)

1. Product Information

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis. 

2. Product Parameter

Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)

                             0.1-1.0EU/ml (assay time about 16 minutes)

3. Product Features and Application

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).

 

Note:

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.

Catalog No.

Description

Kit Contents

Sensitivity EU/ml

EC80545

Bioendo™ EC Endotoxin Test Kit

(End-point Chromogenic Assay,

Diazo Coupling),

80 Tests/Kit

5 Lyophilized Amebocyte Lysate, 1.7ml/vial;

4 Water for BET, 50ml/vial;

5 CSE;

5 Chromogenic Substrate, 1.7ml/vial;

5 Diazo Reagent 1, 10ml/vial;

5 Diazo Reagent 2, 10ml /vial;

5 Diazo Reagent 3, 10ml/vial;

0.1 – 1 EU/ml

EC80545S

0.01 – 0.1 EU/ml;

0.1 – 1 EU/ml

Product Conditions

The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.

 

Does the End point endotoxin test kit need sophisticated microplate reader?

Bioendo EC80545 and EC80545S, can read by regular spectrophotometer. 

Bioendo End point chromogenic endotoxin test kit series:
Endotoxin Free Tube
Pyrogen Free Tips
Pyrogen Free Microplates
normal Microplate reader
Water Bath or Dry Heat Incubator 

Product detail pictures:

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures


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Our firm aims to operating faithfully, serving to all of our shoppers , and working in new technology and new machine regularly for Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: kazakhstan, Czech republic, Saudi Arabia, For more than ten years experience in this filed, our company has gained high reputation from home and abroad. So we welcome friends from all over the world to come and contact us, not only for business, but also for friendship.
  • Sales manager is very enthusiastic and professional, gave us a great concessions and product quality is very good,thank you very much!
    5 Stars By Liz from Mumbai - 2018.06.12 16:22
    This company can be well to meet our needs on product quantity and delivery time, so we always choose them when we have procurement requirements.
    5 Stars By Denise from Greek - 2017.08.21 14:13

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