Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo
Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
By using a full scientific excellent administration method, great quality and fantastic religion, we get good reputation and occupied this discipline for Chinese Professional Chromogenic assays - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Boston, Philippines, Denver, We strongly believe that technology and service is our base today and quality will create our reliable walls of future. Only we've got better and better quality , could we achieve our customers and ourselves, too. Welcome customers all over the word to contact us for getting further business and reliable relationships. We have been always here working for your demands whenever you require.
By Gemma from Lebanon - 2018.06.18 17:25
The factory technical staff gave us a lot of good advice in the cooperation process, this is very good, we are very grateful.
By Eileen from Sweden - 2018.02.12 14:52
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