High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo


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High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo Detail:

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)

1. Product Information

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis. 

2. Product Parameter

Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)

                             0.1-1.0EU/ml (assay time about 16 minutes)

3. Product Features and Application

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).

 

Note:

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.

Catalog No.

Description

Kit Contents

Sensitivity EU/ml

EC80545

Bioendo™ EC Endotoxin Test Kit

(End-point Chromogenic Assay,

Diazo Coupling),

80 Tests/Kit

5 Lyophilized Amebocyte Lysate, 1.7ml/vial;

4 Water for BET, 50ml/vial;

5 CSE;

5 Chromogenic Substrate, 1.7ml/vial;

5 Diazo Reagent 1, 10ml/vial;

5 Diazo Reagent 2, 10ml /vial;

5 Diazo Reagent 3, 10ml/vial;

0.1 – 1 EU/ml

EC80545S

0.01 – 0.1 EU/ml;

0.1 – 1 EU/ml

Product Conditions

The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.

 

Does the End point endotoxin test kit need sophisticated microplate reader?

Bioendo EC80545 and EC80545S, can read by regular spectrophotometer. 

Bioendo End point chromogenic endotoxin test kit series:
Endotoxin Free Tube
Pyrogen Free Tips
Pyrogen Free Microplates
normal Microplate reader
Water Bath or Dry Heat Incubator 

Product detail pictures:

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures

High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo detail pictures


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Our workforce through professional training. Skilled professional knowledge, solid sense of service, to fulfill the services demands of consumers for High definition Chromogenic Endotoxin Testing – Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Ecuador, Bahamas, Ukraine, We look forward to hearing from you, whether you are a returning customer or a new one. We hope you will find what you are looking for here, if not, please contact us immediately. We pride ourselves on top notch customer service and response. Thank you for your business and support!
  • Products and services are very good, our leader is very satisfied with this procurement, it is better than we expected,
    5 Stars By Norma from Surabaya - 2018.06.19 10:42
    As an international trading company, we have numerous partners, but about your company, I just want to say, you are really good, wide range, good quality, reasonable prices, warm and thoughtful service, advanced technology and equipment and workers have professional training, feedback and product update is timely, in short, this is a very pleasant cooperation, and we look forward to the next cooperation!
    5 Stars By Polly from French - 2017.02.28 14:19

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