KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay is significant method for samples with some interference.)
The kinetic chromogenic endotoxin test (KCT or KCET) assay is a method used to detect the presence of endotoxins in a sample.
Endotoxins are toxic substances found in the cell walls of certain types of bacteria, including gram-negative bacteria such as Escherichia coli and Salmonella. In the KCET assay, a chromogenic substrate is added to the sample, which reacts with any endotoxins present to produce a color change.
The rate of color development is monitored over time using a spectrophotometer, and the amount of endotoxin in the sample is calculated based on this rate.
The KCT assay is a popular method for detecting endotoxins in pharmaceuticals, medical devices, and other products that come into contact with the human body. It is a sensitive and reliable test that can detect even very small amounts of endotoxin, making it an important tool for ensuring the safety of these products.
TAL/LAL reagent is the lyophilized amebocyte lysate which is extracted from the blue blood of Limulus polyphemus or Tachypleus tridentatus.
Endotoxins are amphiphilic lipopolysaccharides (LPS) located in the outer cell membrane of gram-negative bacteria. Parenteral products contaminated with pyrogens including LPS can lead to the development of fever, induction of inflammatory response, shock, organ failure and death in human.
Therefore, countries around the world have formulated regulations, requiring that any drug product claiming to be sterile and non-pyrogenic should be tested prior to release. Gel-clot TAL assay was first developed for bacterial endotoxins test (i.e. BET).
However, other more advanced methods of the TAL assay have emerged. And these methods will not only detect but also quantify the presence of endotoxins in a sample. Apart from gel-clot technique, techniques for BET also contain the turbidimetric technique and the chromogenic technique. Bioendo, dedicated to endotoxin detection, is the professional manufacturer to actually develop a chromogenic TAL/LAL assay.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) provides a fast measurement for endotoxin quantification.
We also provide Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) and incubation microplate reader ELx808IU-SN, which could ensure the reliability and efficiency of your experiments.
What is the features of the kinetic chromogenic endotoxin test assay to test endotoxins in the samples?
The kinetic chromogenic endotoxin test assay is another method used to test for endotoxins in samples. It has several features:
1. Kinetic measurement: Similar to the turbidimetric assay, the kinetic chromogenic assay also involves a kinetic measurement. It relies on the reaction between endotoxins and a chromogenic substrate to produce a colored product. The change in color intensity over time is monitored, allowing for the quantification of endotoxin concentrations in the sample.
2. High sensitivity: The kinetic chromogenic assay is highly sensitive and can detect low levels of endotoxins in samples. It can accurately measure endotoxin concentrations, even at very low levels, ensuring reliable detection and quantification.
3. Wide dynamic range: The assay has a wide dynamic range, allowing for the measurement of endotoxin concentrations across a broad spectrum. This means it can test samples with varying levels of endotoxins, accommodating both low and high concentrations without the need for sample dilution or concentration.
4. Rapid results: The kinetic chromogenic assay provides rapid results compared to traditional methods. It typically has a shorter assay time, enabling faster testing and analysis of samples. The color development can be monitored in real-time, and the results can often be obtained within a few minutes to a couple of hours, depending on the specific assay kit and equipment used.
5. Automation and standardization: The assay can be performed using automated systems, such as microplate readers or
endotoxin-specific analyzers. This allows for high-throughput testing and ensures consistent and standardized measurements, reducing human error and increasing efficiency.
6. Compatibility with various sample types: The kinetic chromogenic assay is compatible with a wide range of sample types, including pharmaceuticals, medical devices, biologics, and water samples. It is a versatile method that can be applied to different industries and applications where endotoxin testing is required.
Overall, the kinetic chromogenic endotoxin test assay offers a sensitive, rapid, and reliable method for detecting and quantifying
endotoxins in samples. It is widely used in pharmaceutical, biotechnology, and healthcare industries for quality control and safety
assessment purposes.
Post time: Jul-29-2019
