Professional China Chromogenic endotoxin test - Endpoint Chromogenic Kit EC80545 – Bioendo
Professional China Chromogenic endotoxin test - Endpoint Chromogenic Kit EC80545 – Bioendo Detail:
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Product Information
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
2. Product Parameter
Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)
0.1-1.0EU/ml (assay time about 16 minutes)
3. Product Features and Application
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 – 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Note:
Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.
|
Catalog No. |
Description |
Kit Contents |
Sensitivity EU/ml |
|
EC80545 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling), 80 Tests/Kit |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Water for BET, 50ml/vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml /vial; 5 Diazo Reagent 3, 10ml/vial; |
0.1 – 1 EU/ml |
|
EC80545S |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Does the End point endotoxin test kit need sophisticated microplate reader?
Bioendo EC80545 and EC80545S, can read by regular spectrophotometer.
Product detail pictures:
Related Product Guide:
With advanced technologies and facilities, strict high-quality handle, reasonable rate, superior services and close co-operation with prospects, we are devoted to furnishing the best price for our customers for Professional China Chromogenic endotoxin test - Endpoint Chromogenic Kit EC80545 – Bioendo , The product will supply to all over the world, such as: Libya, Canada, Monaco, As operation principle is "be market-oriented , good faith as principle, win-win as objective", holding on "customer first, quality assurance, service first" as our purpose, dedicated to provide the original quality, create excellence service , we won the praise and trust in the industry of auto parts. In the future, We will provide quality product and excellent service in return to our customers , welcome any suggestions and feedback from all over the world.
By Christine from Madagascar - 2018.06.19 10:42
Good quality, reasonable prices, rich variety and perfect after-sales service, it's nice!
By Janet from Bandung - 2017.07.07 13:00
Leave Your Messages
Related products
-
Wholesale Kinetic chromogenic LAL test kit - E...
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) 1. Product Information Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum abso...
-
Best quality Kinetic Turbidimetric LAL Test - ...
Bioendo KT Endotoxin Test Kit (Kinetic Turbidimetric Assay) 1. Product Introduction Kinetic Turbidimetric Amebocyte Lysate Vial is developed based on the principle that the time needed to reach a certain absorbance increase (onset OD), i.e. onset time, is negatively correlated with the endotoxin concentration. Sensitivity could reach 0.005EU/ml, and the detection could reach four orders of magnitude. It is specially suitable for pharmaceuticals industry to monitor endotoxin concentration. T...
-
High reputation 1000ul Pipette tip box - Nymph...
NYMPHX Nymph X Water Purification System Nymph X Water Purification System could transfer tap water into pure water and ultrapure water. Equipped with a pressure stabilizer and low flow-rate operating mode, the system can adapt to various source conditions without any extra pre-treatment necessary. The system also supports multiple water dispensing modes, and the precision of quantitative and quality-controlled dispense of water could reach ±1%. Meanwhile, comprehensive monitoring could be...
-
Super Lowest Price Endotoxin Free Microplate -...
Control Standard Endotoxin (CSE) 1. Product Information Control Standard Endotoxin (CSE) is extracted from E.coli O111:B4. CSE is an economic alternative to Reference Standard Endotoxin (RSE) in constructing standard curves, validating product and preparing controls in Lyophilized Amebocyte Lysate test. The labeled potency of CSE endotoxinE.coli standard is referenced against RSE. The Control Standard Endotoxin could be used with gel clot assay, kinetic turbidimetric assay or kinetic chromog...
-
OEM/ODM China Rapid Endotoxin Detection - Endo...
Endotoxin Challenge Vials (Endotoxin Indicator) 1. Product Information The Endotoxin Challenge Vial (ECV，Endotoxin Indicator) is used in the validation of dry heat depyrogenation cycles. Endotoxin Challenge Vials are placed in the cold spots of the dry heat oven. After cycle completion, the log reduction in endotoxin levels can be determined by comparing the endotoxin levels in the baked vs not baked Endotoxin Indicators. The Endotoxin Challenge vials are designed to indicate a minimum 3...
-
Manufactur standard Endotossine LAL Test - End...
Endotoxin and (1,3)-ß-D-glucan assay software 1. Product information Endotoxin and (1,3)-ß-D-glucan assay software is a powerful kinetic data analysis software,which gives the user data acquisition and processing of the maximum flexibility. Features: • Apply to Endotoxin assay,（1,3）-ß-D-glucan assay and ELISA data analysis • With standard version and clinical diagnostic version for the different user groups. • Data could be output transitions and connected to the LIS system. • Customizabl...
